Conductivity and Viscosity Measurements for Binary Lysozyme Chloride Aqueous Solution and Ternary Lysozyme-Salt-Water Solution

摘要

We use the conductimetric method, adequate to electrolytes, to determine thelysozyme charge in lys-water and ternary lys-salt-water systems. We measuredalso the viscosities for the above binary and ternary systems in the sameconditions at pH$=4.5$ and T$=298$ K, measurements that allow us to see anyeffect of viscosity on cations mobilities and implicitly on the lysozymecharge. The method is illustrated for the lysozyme chloride aqueous solutionsystem at 25$^o$ C, using the data reported here for pH$=4.5$ at 0.15, 0.6,0.8, 1., 1.5, 2., 2.5, 3., 3.5 mM (mg/mL) lysozyme chloride concentrations. Themethod was also applied to ternary lys-salt-water systems in the sameconditions at pH$=4.5$ and T$=25^o$ C. Ternary conductivities are reported fora mean concentration 0.6 mM of lysozyme chloride in all systems and a meanconcentration 0.01, 0.025, 0.05, 0.1, 0.175, 0.2, 0.5, 0.7, 0.9, 1.2, 1.3 and1.4 M for NaCl; 0.005, 0.01, 0.05, 0.1, 0.175, 0.2, 0.5, 0.7, 0.9, 1.2, 1.3,1.4 and 1.5 M for KCl; 0.005, 0.01, 0.05, 0.1, 0.175, 0.2, 0.5, 0.7, 1.2, 1.3,1.4, 1.5, 1.6 and 1.7 M for NH$_4$Cl.